TEAD1在小鼠急性心肌梗死后心肌组织中的表达作用
张 驰 （ 宁夏医科大学临床医学院 ）
燕 茹 （ 宁夏医科大学总医院心脏中心 ）
贾绍斌 （ 宁夏医科大学总医院心脏中心 宁夏医科大学 宁夏医科大学总医院医学科学研究院 ）
https://doi.org/10.37155/2661-4766-0506-62Abstract
目的： 观察TEA转录因子1（ TEAD1）在小鼠急性心肌梗死后心肌组织中的表达情况及细胞定位，进 一步探讨TEAD1在急性心肌梗死后作用及相关机制。 方法： 将40只6周龄C57BL/6雄性小鼠随机分为急性心肌梗死组 （ AMI组）和假手术组（ Sham组）， AMI组通过结扎冠状动脉左前降支近端的方法建立急性心肌梗死模型，造模成 功以观察即刻心电图ST段改变判断， Sham组仅用6-0手术线穿过冠状动脉左前降支近端但不结扎。两组分别于术后 1、 3、 7、 14天取材，进行HE染色及Masson染色观察急性心肌梗死后心肌组织的病理改变。免疫组化检测转录因子 TEAD1和巨噬细胞标志物CD68在急性心肌梗死后小鼠心肌组织中的表达情况；免疫荧光双标法观察TEAD1在急性心 肌梗死后心肌组织中是否在巨噬细胞中高表达。 结果： TEAD1免疫组化结果显示， Sham组和AMI组在不同时间均在 心肌细胞核可见TEAD1少量表达；与同时间点Sham组相比， AMI组各时间点TEAD1表达量明显增加（ P均 < 0.01）， AMI组各时间点TEAD1表达呈先增后减的变化趋势，且主要在梗死交界区表达，其中AMI组第3天时表达量最多， 且AMI组相邻各时间点TEAD1表达量之间有明显统计学差异（ P均 < 0.001）； CD68免疫组化结果显示，与同时间点 Sham组相比， AMI组各时间点CD68表达量明显增加（ P均 < 0.001）， AMI组各时间点CD68表达呈先增后减的变化趋 势，且主要在梗死交界区表达，其中AMI组第3天时表达量最多，且AMI组相邻各时间点CD68表达量之间有明显统计 学差异（ P均 < 0.001）；免疫荧光双标结果显示TEAD1在AMI组心肌组织中的巨噬细胞中高表达，除第14天外，与同 时间点Sham组相比，表达TEAD1的巨噬细胞数量在AMI组各时间点明显增加（ P均 < 0.05），表达TEAD1的巨噬细胞 数量在AMI组各时间点呈先增后减的变化趋势，且主要在梗死交界区表达，其中AMI组第3天时表达量最多，且AMI 组相邻各时间点表达TEAD1的巨噬细胞数量之间有明显统计学差异（ P均 < 0.01）。 结论： 转录因子TEAD1和巨噬细 胞标志物CD68在小鼠急性心肌梗死后心肌组织中都呈现表达增加的特点，在急性心肌梗死后第3天表达量最多，并且 主要在梗死交界区表达； TEAD1除了心肌细胞表达外，可以定位在急性心肌梗死后梗死交界区的巨噬细胞中。
Keywords
TEAD1；急性心肌梗死；巨噬细胞；小鼠Full Text
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Copyright © 2024 张 驰,燕 茹,贾绍斌
Publishing time:2023-12-31
This work is licensed under a Creative Commons Attribution 4.0 International License
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