肠道微生物群与胃食管反流病的因果关系:两样本孟德尔随机化分析

周嘉伟 ( 暨南大学附属广东省第二人民医院 )

李晓锋 ( 暨南大学附属广东省第二人民医院 )

李海量 ( 暨南大学附属广东省第二人民医院 )

杨 倜 ( 暨南大学附属广东省第二人民医院 )

黎 程 ( 暨南大学附属广东省第二人民医院 )

https://doi.org/10.37155/2661-4766-0603-1

Abstract

目的:利用孟德尔随机化(Mendelian randomization,MR)分析研究肠道微生物群与胃食管反流病 (Gastroesophageal reflux disease,GERD)的因果关系,为研究GERD的发病机制及其潜在的预防和治疗策略提供遗 传学视角。方法:筛选数据后使用逆方差加权法(inverse variance weighted,IVW)、加权众数法(weighted mode estimate)、模型选择法(MR-Egger)和加权中位数法(weighted median estimate)进行MR分析;再行敏感性分析和 反向MR分析,从而确保结果的有效性。结果:两样本孟德尔随机化(two-sample Mendelian randomization,TSMR) 分析结果显示8类细菌与GERD相关,包括4种危险因素和4种保护因素。GERD的风险与双歧杆菌科、双歧杆菌目、克 里斯滕森菌科、臭杆菌属呈负相关,与柔膜体纲、软壁菌门、理研菌科和普雷沃氏菌属9呈正相关;敏感性分析未发 现异质性和水平多效性,反向MR分析未发现肠道菌群与GERD之间存在反向因果关系。结论:通过TSMR,我们确立 了肠道菌群(8类)与GERD之间的因果关系,从而为研究GERD发病机制及其预防和治疗的潜在策略提供了创新性的 遗传学观点。

Keywords

孟德尔随机化;肠道菌群;胃食管反流病;因果关系

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