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Preparation and Physicochemical Evaluation of Hydroxyapatite-gelatin Nanoparticles

Kivanc Ozaslan （ Department of Industrial Engineering, Faculty of Engineering, Hacettepe University, Ankara, Beytepe, 06800, Turkey. ）

Emre Bozkaya （ Department of Industrial Engineering, Faculty of Engineering, Hacettepe University, Ankara, Beytepe, 06800, Turkey. ）

https://doi.org/10.37155/2717-526X-0401-3

Abstract

One of the promising fields of research is tissue engineering that can facilitate available therapeutic methods. Nanosized hydroxyapatite (HA) is one of the key elements in mineral bone. It is possible to affect the surrounding osteoprogenitor cells using nano-sized HA and through this improve bone repair through changing paracrine signaling. The present paper is an attempt to prepare and evaluate physicochemical properties of hydroxyapatite-gelatin nanoparticles. To this end, two sizes were prepared including S100 and S150 using standard chemical precipitation. To characterize the size and morphology of the synthesized powders, X-ray diffraction and Brunauer-Emmett-Teller (BET) surface area were determined using Autosrob-IQ2-MP. To measure the calcium ions released by HANPs, an inductively coupled plasma optical emission spectrometer (ICP-OES) was used. The collected data was analyzed in SPSS 19.0. In the case of S100, the hydrodynamic diameter based on DLS analyses was equal to 626.10±14.95nm; this figure for S150 was equal to 262.33±46.5. There was a larger specific surface area in S100 compared to S150; in addition, S100 had wider diffraction peaks, which is in agreement with small and poorly crystalline crystals. On the other hand, the diffraction peaks of S150 were sharper, which means that the crystallinity was higher in S150. In addition, HANPs of all sizes had degradability and HANPs with smaller sizes (S100) degraded faster compared to larger-sized S150. The pH level of the control, S100, and S150 was 7.24±0.01, and 7.26±0.02 so that there was no significant difference between them. Nanoparticle size is a key factor in the biological environment, which provides a reference for HANPs in biomedical uses.

Keywords

Hydroxyapatite-gelatin; Nanoparticles; Nanotechnology

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References

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Copyright © 2022 Emre Bozkaya, Kivanc Özaslan Creative Commons License Publishing time:2022-07-08
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