酶法制备6-APA中的水解工序中影响因素确认
陈 海 （ 山西双雁药业有限公司 ）
刘学俊 （ 山西双雁药业有限公司 ）
https://doi.org/10.37155/2717-5669-0607-14简介
6-氨基青霉烷酸（英文书写为6-Aminopenicillanic acid，简称6-APA)作为青霉素中间体的代表，是目前 原料药市场上一种重要的医药中间体，在抗生素生产中具有不可替代的作用。本文旨在对青霉素水解酰化酶在制备生 产6-APA合成中时对控制环境温度、反应的pH值、参与反应的底物浓度等对催化反应的影响方面进行研究。
关键字
青霉素水解酰化酶、酶水解、6-氨基青霉烷酸全文
PDF参考文献
[1] Duggleby H.J., Tolley S.P., Hill C.P., et al. Penicillin
acylase has a single amino acid catalytic centre[J]. Nature,
1995, 373(6511): 264-268
[2] Schumacher G., Sizmann D., Haug H., et al.
Penicillin acylase from E. coli: unique gene-protein
relation[J]. Nucleic Acids Research, 1986, 14(14): 5713-5727
[3]Mcvey C.E., Walsh M.A., Dodson G.G., et al. Crystal
structures of penicillin acylase enzyme-substrate complexes:
structural insights into the catalytic mechanism[J]. Journal of
Molecular Biology, 2001, 313(1): 139-150
[4] Suresh C.G., Pundle A.V., Sivaraman H., et al.
Penicillin V acylase crystal structure reveals new Ntnhydrolase family members[J]. Nature Structural Biology,
1999, 6(5):414-416
[5] Grulich M., Štěpánek V., Kyslík P. Perspectives and
industrial potential of PGA selectivity and promiscuity[J].
Biotechnology Advances, 2013, 31(8): 1458-1472
[6]崔鹏、万敏 青霉素酰化酶的生产与应用新进展 化
学工业与工程技术2005年 第六期 第42卷 第6页
[7] 陈坚,固定化青霉素酰化酶水解青霉素 G的研究 无
锡轻工大学学报 1995年 第二期 第14卷 第5~7页
[8] Bahamondes C., Wilson L., Aguirre C., et al.
Comparative study of the enzymatic synthesis of cephalexin
at high substrate concentration in aqueous and organic media
using statistical model[J]. Biotechnology and Bioprocess
Engineering, 2012, 17(4): 711-721
[9] Illanes A., Wilson L., Corrotea O., et al. Synthesis of
cephalexin with immobilized penicillin acylase at very high
substrate concentrations in fully aqueous medium[J]. Journal
of Molecular Catalysis B Enzymatic, 2007, 47(1-2): 72-78
acylase has a single amino acid catalytic centre[J]. Nature,
1995, 373(6511): 264-268
[2] Schumacher G., Sizmann D., Haug H., et al.
Penicillin acylase from E. coli: unique gene-protein
relation[J]. Nucleic Acids Research, 1986, 14(14): 5713-5727
[3]Mcvey C.E., Walsh M.A., Dodson G.G., et al. Crystal
structures of penicillin acylase enzyme-substrate complexes:
structural insights into the catalytic mechanism[J]. Journal of
Molecular Biology, 2001, 313(1): 139-150
[4] Suresh C.G., Pundle A.V., Sivaraman H., et al.
Penicillin V acylase crystal structure reveals new Ntnhydrolase family members[J]. Nature Structural Biology,
1999, 6(5):414-416
[5] Grulich M., Štěpánek V., Kyslík P. Perspectives and
industrial potential of PGA selectivity and promiscuity[J].
Biotechnology Advances, 2013, 31(8): 1458-1472
[6]崔鹏、万敏 青霉素酰化酶的生产与应用新进展 化
学工业与工程技术2005年 第六期 第42卷 第6页
[7] 陈坚,固定化青霉素酰化酶水解青霉素 G的研究 无
锡轻工大学学报 1995年 第二期 第14卷 第5~7页
[8] Bahamondes C., Wilson L., Aguirre C., et al.
Comparative study of the enzymatic synthesis of cephalexin
at high substrate concentration in aqueous and organic media
using statistical model[J]. Biotechnology and Bioprocess
Engineering, 2012, 17(4): 711-721
[9] Illanes A., Wilson L., Corrotea O., et al. Synthesis of
cephalexin with immobilized penicillin acylase at very high
substrate concentrations in fully aqueous medium[J]. Journal
of Molecular Catalysis B Enzymatic, 2007, 47(1-2): 72-78
版权所有 © 2025 陈 海,刘学俊
出版时间:2025-07-31
本作品采用以下许可协议授权： 知识共享 署名 4.0 国际许可协议
